Look up human growth hormone on the internet. a) How many amino acids long is the human growth hormone? b) What is the minimum length in nucleotides for the mRNA that encodes growth hormone? c) Say you want to produce recombinant growth hormone for medical use. You place the human growth hormone gene into the bacteria E. coli. However, the E. coli don't produce any growth hormone. List two reasons the human gene may not be expressed in E. coli.
Added by Nicole S.
Step 1
b) The minimum length in nucleotides for the mRNA that encodes growth hormone would be 3 times the number of amino acids, as each amino acid is encoded by a triplet of nucleotides (codon). So, the minimum length would be 191 amino acids x 3 nucleotides = 573 Show more…
Show all steps
Your feedback will help us improve your experience
Sri K and 98 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
Explain how you might engineer $E$. coli to produce human growth hormone (HGH) using the following: E. coli containing a plasmid, DNA carrying the gene for HGH, DNA ligase, a restriction enzyme, equipment for manipulating and growing bacteria, a method for extracting and purifying the hormone, and an appropriate DNA probe. (Assume that the human HGH gene lacks introns.)
What can be done to make a lot of human growth hormone so that it can be used therapeutically? A. Clone the gene into bacteria and harvest it from them. B. Clone the human growth hormone gene into chickens and harvest it from the eggs. C. Put the growth hormone genes into tomatoes and have people eat them. D. Use gene therapy to put the gene into people who need more growth hormone.
Joy C.
You want to investigate the function of a set of human proteins and decide to express the proteins in E. coli first for in vitro studies. Thus, you need to get the genes encoding proteins of interest by PCR, using cDNA as a template. a. What is the difference between human genomic DNA and cDNA? [Hint: Chap. 7.10. Cloning Complementary DNA Avoids Introns] b. Why can't you use human genomic DNA as a PCR template to obtain the gene for expression in bacteria? [Hint: two problems.] c. You decide to make a cDNA library. What will you do to isolate messenger RNA from human cells?
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD