Make a diagram to explain how a human gene can be cloned into a bacterial plasmid. You should represent the events that need to occur in order to: Clone the insulin gene into a bacterial plasmid, Transform the plasmid into E. coli Include definitions or descriptions of the following terms/components: Restriction enzyme(s) Ligase Plasmid DNA Human mRNA (the mRNA for insulin) Reverse transcriptase Transformation E. coli marker genes (an antibiotic resistance gene) Cloning vector
Added by Luisa F.
Step 1
First, we need to isolate the human mRNA for insulin. This can be done by extracting mRNA from human pancreatic cells. Show more…
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The diagram below shows a plasmid with a human gene inserted (grey). Add labels to the diagram to show all of the features you would need to include in the plasmid so that (1) the plasmid can be successfully transformed into a bacteria, (2) the plasmid can be replicated, and (3) the expression vector can be used to synthesize proteins from this gene sequence How would the sequence of this gene be different than the sequence of the same gene found in the human genome? How was the gene inserted into the plasmid?
Sri K.
Draw a diagram/figure to explain the conjugation process (e.g. use PowerPoint or draw one by hand and include a photo of it). You should include in the diagram the F- recipient, Hfr Donor, and the transconjugant/recombinant recipient. Make sure to include the genes encoding for Leucine, Threonine, Thiamine, and Streptomycin resistance in your diagram. How does an Hfr strain of E. coli transfer chromosomal DNA to an F- strain? What determines how much of the chromosomal DNA is transferred?
Supreeta N.
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