Figure 4. Minimal media for bacterial cultures. You will grow transformants in a defined media solution with Kanamycin (an antibiotic to select for transformants) and lactose (to induce expression of the gene inserted into pAU21). The final concentrations of media components are shown in Table 1:
Table 1. Final Growth Media Concentrations
Media Component Final Conc
M9 Salts 1x
Glucose 0.11 M
Calcium Chloride 0.1 mM
Lactose 0.05 M
Kanamycin 50 μg/ml
You have the following stock solutions to make your media (Table 2):
Table 2. Stock Solution Concentrations
Media Component Stock Solution
M9 Salts 200x
Glucose 2 M
Calcium Chloride 1 M
To make 1000 ml of media, how many milliliters of the stock 200x concentration of M9 salts should be added?
a. 50 ml
b. 5 ml
c. 10 ml
d. 0.5 ml
e. 1 ml
To make 1000 ml of media, how many milliliters of the stock 2 M Glucose (MW 180.16 g/mol) should be added?
a. 55 ml
b. 25 ml
c. 27.5 ml
d. 110 ml
e. 5.5 ml
To make 1000 ml of media, how many milliliters of the stock 1 M Calcium Chloride (MW 110.98 g/mol) should be added?
To make 1000 ml of media, how many grams of Lactose (MW 342.3 g/mol) should be added?
To make 1000 ml of media, how many milligrams of Kanamycin should be added?