Make Bacterial Media
Figure 4. Minimal media for bacterial cultures
You will grow transformants in a defined media solution with Kanamycin (antibiotic to select
for transformants) and lactose (to induce expression of gene inserted into pAU21). The final
concentrations of media components are (Table 1):
Table 1. Final Growth Media Concentrations
Media
Component
Final Conc
M9 Salts
1x
Glucose
0.11 M
Calcium Chloride
0.1 mM
Lactose
0.05M
Kanamycin
50 ug/ml
You have the following stock solutions to make your media (Table 2):
Table 2. Stock Solution Concentrations
Media
Component
Stock Solution
M9 Salts
200x
Glucose
2M
Calcium Chloride
1M
To make 1000 ml of media, how many milliliters of stock 200x concentration of M9 salts
should be added?
a. 50 ml b. 5 ml c. 10 ml d. 0.5 ml e. 1 ml
- To make 1000 ml of media, how many milliliters of stock 2M Glucose (MW 180.16 g/mol)
should be added?
a. 55 ml b. 25 c. 27.5 ml d. 110 ml e. 5.5 ml
- To make 1000 ml of media, how many milliliters of stock 1M Calcium Chloride
(MW 110.98 g/mol) should be added?
- To make 1000 ml of media, how many grams of Lactose (MW 342.3 g/mol) should be
added?
To make 1000 ml of media, how many milligrams of Kanamycin should be added?
