Match each genetic component involved with GAL gene regulation in yeast with the best description of its function. Enhancer element upstream of gal genes Transcription factor with a DNA binding domain and trans-activation domain Protein that interacts with GAL4 when no galactose is present Protein that senses the presence of galactose DNA binding protein that leads to repression of transcription in response to glucose
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Step 2: The transcription factor with DNA binding domain and trans-activation domain is GAL4, which is a key regulator of gal gene expression. Step 3: The protein that interacts with GAL4 when no galactose is present is GAL80, which inhibits GAL4 activity in the Show more…
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Yeast genes have $c$ is-acting elements upstream of their promoters, similar to enhancers, called upstream activating sequences or UASs. Several target genes in- volved in galactose utilization are regulated by one type of UAS called $U A S_{G}$ which has four binding sites for an activator called GAL4. Two target genes regulated by $U A S_{G}$ are $G A L 7$ and $G A L I O$. The GAL 80 protein is an indirect repressor of $G A L 7$ and $G A L I O$ transcription: At $U A S_{C}$ GAL80 binds to GAL4 protein and blocks GAL4's activation domain. In the presence of galactose, GAL 80 no longer binds GAL 4 In which gene(s) $(G A L 4 \text { and/or } G A L 80$ ) should you be able to isolate mutations that allow the constitutive expression of the target genes $G A L 7$ and $G A L I O$ in the absence of galactose? In each case, what characteristics of the protein would the mutation disrupt?
Although operons are not common in eukaryotes, eukaryotic genes may be activated by the same stimulus. Which of the following DNA regulatory sequence makes this coordinated gene expression possible? Core promoter Enhancer element Response element Boundary element Silencer element QUESTION 18 A mutation in the gene for the yeast regulatory protein GAL4 causes yeast to grow poorly on galactose. What is the function of GAL4? It is a substrate that binds and activates a transcriptional activator. It is a product that binds and activates a transcriptional repressor. It is a transcription activator for the galactose-digesting enzyme gene. It is a transcription repressor that prevents expression of yeast galactose-digesting enzymes. It is an enzyme that metabolizes galactose.
Adi S.
In details describe the multiple ways in which genes are regulated in eukaryotes - describe how each of the following increases or decreases gene expression: specific histone modifications, DNA methylation, basal transcription factors, transcriptional activator proteins binding at enhancers, and RNA splicing (indicate if gene regulation is increased or decreased by each mechanism). Next, briefly describe at what level gene regulation mainly occurs in bacteria.
Dominador T.
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