00:01
So match the correct concentration of cell to the image to complete the zero dilution scheme shown here.
00:07
So you start out with the original culture, which has a concentration of 100 ,000 cells per ml.
00:16
Now the first step, you take out 1 ml from the original stock and add it into 9.
00:23
So in this case, you have 1 ml to begin with.
00:27
You end up with 1 plus 9, which is 10 ml.
00:29
So this means that this is a 1 to 10 dilution.
00:34
The concentration drops 10 times.
00:38
So let me write down here, 1 to 10 dilution, and the concentration drops 10 times.
00:56
So 100 ,000 divided by 10 equals 10 ,000.
01:05
So this is the first tube.
01:09
The second tube, you repeat this process.
01:10
You take 1 out of the second tube and then dilute it to 9 ml of diluent.
01:16
This is again a 1 to 10 dilution.
01:18
However, the total dilution is going to be 1 to 10 times previous 1 to 10.
01:25
So it's a 1 to 100 in total dilution...