Match the definition with the parameters of microscopy. 1....

Match the definition with the parameters of microscopy. 1. (Click to select) Related to the clarity of the image through the microscope. This allows being able to distinguish between two separate structures. 2. (Click to select) The visualization of an object, sometimes aided with dyes and stains to create differences between structures. 3. (Click to select) Identifying the ratios between the actual size of an object and the image viewed through a microscope. es 4. (Click to select) Not a parameter of microscopy, but rather a type of microscopy.

Transcript

00:01 Hello everyone, in this question we need to convert between the metric size units of meter, centimeter, millimeter, microns and nanometer.

00:10 So here 1 meter is equals to 100 centimeters and 1 meter is equals to 1000 millimeters and 1 meter is equals to 10 to the power 6 microns and 1 meter will be equals to 10 to the power 9 nanometer.

00:33 Then if you convert it to centimeter, the 1 centimeter here will be equals to 10 to the power minus 2 meters and it will be equals to 10 millimeters, it will be equal to 1000, so 10 ,000 microns and 1 centimeter is equals to 10 to the power 7 nanometer.

00:58 Then 1 millimeter is equals to 10 to the power minus 3 meters, then 1 millimeter is equals to 10 to the power minus 1 centimeter, it is equals to 1000 microns and equals to 10 to the power 6 nanometer.

01:20 Then 1 micron will be equals to 10 to the power minus 6 meters and it will be equals to 10 to the power minus 4 centimeters and it will be equals to 1 to the 10 to the power minus 3 millimeter and equals to 1000 nanometers.

01:45 Then 1 nanometer is equals to 10 to the power minus 9 meters and it is equals to 10 to the power minus 7 meters and 10 to the power minus 6 millimeter and 10 to the power minus 3 microns.

02:03 Then we have been asked to describe the basic difference between the light microscopy, dark field phase contrast and electron microscopy and scanning electron microscopy.

02:16 So, here let us write light microscopy and in this we will write the electron microscope.

02:28 So, the light microscopy uses the light for illuminating the object, this here uses the electron beam as illuminating source, it produces lower magnification and the electron microscopy produces the higher magnification.

02:52 The light microscopy resolution is low, the electron microscopy produces high resolution as the electrons have shorter wavelength than the visible light and it can be used to visualize both the living as well as the dead specimen.

03:18 The electron microscopy are placed under the vacuum and therefore, the live specimen cannot be viewed only the dead and dried samples can be viewed.

03:30 Lengths of light microscopy are made above the glass whereas that of the electron microscopy it is made above electromagnet.

03:41 In this the specimen preparation is easy in comparison to the electron microscope, this here is a tedious process and corrosive chemicals are used.

03:56 In this the image is viewed from the ocular lens or known as the eyepiece...

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