00:01
Hi, in this question we have to talk about the enzymes.
00:05
We are given several, you can say, some parts of this question.
00:09
In first part, we are asked about the nucleotide that joins in the sequence complementary to the dna template.
00:17
So i must tell you that if we talk about this enzyme, it will be called as the dna polymerase.
00:26
And if we talk about this dna polymerase, you must understand that this enzyme is very much important to add the nucleotides to an existing nucleic acid strand.
00:37
And it cannot add these nucleotides without an, you can say, existing nucleic acid.
00:45
And that is why it will require a primer.
00:50
Fine.
00:51
This is the first part of the question.
00:53
The second part of the question asks about the enzyme that joins the nucleotides so that the sequence is complementary to the parental strand.
01:03
And to this, the answer is the dna ligase.
01:08
Okay, so we'll write dna ligase over here as answer.
01:13
If we talk more about this, then this dna ligase is important to form the phosphodiceter bond.
01:22
This phosphodicell bone is very important to bond the strands of the dna.
01:28
Yes, so it will connect the two strands we can say of the double stranded dna.
01:37
Okay.
01:37
And also you must understand that it will also link the okazaki fragments.
01:44
What are these okazaki fragments? these okazaki fragments are the lagging strand.
01:52
Why lagging strand? because you have to attach the primer again and again.
01:58
Okay? because the fork that is formed during the replication of dna has to be opened again and again by the helicase enzyme.
02:06
Okay.
02:07
So this is the dna like case.
02:08
That is the second part of the question.
02:10
Let us just mark it as the answer of the second part.
02:14
And the first part answer is the dna polymerase that we have marked over here...