Match the form of microscopy to its description. transmission electron scanning electron white light Fluorescent light A. Narrow wavelength light shines down on a specimen and labeled antibodies, dyes or fluorescent proteins glow. B. Electrons pass through a thin section of specimen and membranes and organelles are visualized. C. Broad wavelength light passes through a specimen and dyes and/or filters reveal certain structures. D. Electrons bounce off a metal-coated specimen and surface features are revealed.
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Narrow wavelength light shines down on the specimen and labeled antibodies, dyes, or fluorescent proteins glow: This description matches with **Fluorescent light** microscopy. Show more…
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Match the type of microscopy with the best corresponding description provided below. Each answer may be used once or not at all. Uses an optical component to take advantage of the different refractive indices of light passing through different regions of the cell. Produces a halo effect around the object. Has the best resolution and greatest magnification but produces a two-dimensional image. Requires the use of two sets of filters. The first filter narrows the wavelength range that reaches the specimen and the second blocks out all wavelengths that pass back up to the eyepiece except for those emitted by the dye in the sample.
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Analyze the following statements to identify which techniques have similarities to scanning electron microscopy (SEM) for the reason given: Choose one or more: SEM and transmission electron microscopy use the same illumination source. SEM and differential interference contrast microscopy both provide 3D images of the cell surface. SEM and the Gram stain technique both use multiple treatments with stains, washes, and mordants to view specimens. SEM and X-ray crystallography both provide the same level of resolution. SEM and differential staining both only use one stain.
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Complete the following statements about microscopy. 5. The ratio of an object's size under a microscope to its real size is ____________. 6. The ____________ of an image is a measure of the clarity of the image or the minimum distance of two distinguishable points. 7. The ____________ of an image is the visible differences in parts of the specimen. 8. The ____________ uses light passing through lenses to magnify specimens. 9. A light microscope can magnify about ____________ times the actual size of the specimen. 10. ____________ electron microscopes pass a beam of electrons through a specimen in order to study the internal structure of the cell. 11. ____________ electron microscopes pass a beam of electrons over the surface of a specimen to produce a three-dimensional image.
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