Matching: 20.) Match each protein on the left with its function/function on the right. Each protein may be used only once. ENZYME A. Helicase B. DNA Polymerase C. Primase D. Single Stranded Binding Proteins E. Leading Strand F. Lagging Strand G. Ligase FUNCTION/ DEFINITION Reads DNA in the 3`to 5`direction, synthesises complementary DNA strands in the 5`to 3`direction Created through discontinuous replication, comprised of Okazaki fragments Functions to unwind the DNA helix at the Origin of Replication Created through continuous replication Functions to lay down RNA primers to allow DNA Polymerase to function Functions to bind Okazaki fragments together Function to stabilize DNA strands when separated Short Answer: Answer the following questions in the spaces provided.
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Step 1:** Match the protein Helicase with its function: Functions to unwind the DNA helix at the Origin of Replication ** Show more…
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Match each enzyme with its corresponding function in bacterial DNA replication: Helicase Single strand binding proteins DNA polymerase III DNA polymerase I DNA ligase Primase Gyrase A. Main "work horse" of DNA replication. Attaches nucleotides to growing DNA strand. B. Creates a short RNA primer. C. Removes primers and replaces with DNA nucleotides. D. Breaks hydrogen bonds, separating DNA strands. E. Topoisomerase that relieves tension created by unwinding DNA. F. Forms phosphodiester bond between two existing DNA strands. G. Prevents formation of secondary structures in the template DNA. QUESTION 2 During DNA replication, new nucleotides are attached to the ________ of the last nucleotide of the growing DNA strand. 5' phosphate group 2' OH group 3' OH group nitrogenous base 3' phosphate group 5' OH group
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Match the following components of a replication fork to the correct description. Question Selected Match Helicase G. unwinds DNA at the replication fork RNA Primase H. polymerase that removes the RNA primer and fills in the gap DNA ligase D. enzyme that seals the nicked DNA backbone DNA polymerase III I. main replication polymerase DNA pol I H. polymerase that removes the RNA primer and fills in the gap Okazaki Fragments F. short sequences of replicated DNA generated during discontinuous strand synthesis Single Strand Binding Proteins A. lays RNA primer for replication Topoisomerase II E. enzyme that unwinds the DNA helix to relieve torsional strain via cutting both strands Leading strand synthesis J. continuous strand synthesis Lagging Strand Synthesis K. discontinuous DNA synthesis Topoisomerase I B. enzyme that unwinds the DNA helix to relieve torsional strain via cutting a single strand only
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Replication A. requires that a phosphodiester bond of the incoming dNTP be hydrolyzed in order to be added to the growing chain. B. uses $5^{\prime}$ to $3^{\prime}$ polymerase activity to synthesize one strand and $3^{\prime}$ to $5^{\prime}$ polymerase activity to synthesize the complementary strand. C. owes its accuracy, in part, to $3^{\prime}$ to $5^{\prime}$ exonucleolytic activity of DNA polymerases or associated proteins. D. begins with two dNTPs joining together. E. requires only proteins with DNA polymerase activity.
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