may sumer Trom DNA contamination, but this is generally not an analytical concern Question 4 Regarding the assessment of extracted nucleic acids, RNA yield can be assessed by determining the 28S/18S rRNA ratio DNA yield is reflected by its absorbance at 280 nm high quality DNA and/or RNA preparations are associated with high molecular weight fragmelits densitometric methods allow the assessment of only nucleic acid yield spectrophotometric methods are based on UV light absorbance by the phosphate backbone of nucleic acid molecules destion 5
Added by Tammy S.
Close
Step 1
None Show more…
Show all steps
Your feedback will help us improve your experience
Shaiju T and 85 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
The 280/260 nm absorbance ratio for nucleic acids is important for determining nucleic acid purity and concentration. It is also important for determining the ratios of A to G and C to T in double-stranded DNA. Contamination with RNA can be determined by measuring the absorbance at 260 nm.
Shaiju T.
A260/A230 is the ratio of nucleic acids to other contaminants, and A260/A280 is the ratio of nucleic acids to proteins. Please analyze the extracted DNA.
Madhur L.
Part 1. What is/ are the aim(s) of quantifying and qualifying your DNA isolate? Pick all that apply. Making sure that your DNA is pure and free of contaminants Making sure that your DNA is intact Making sure that you have enough DNA for any downstream processes Making sure that your students have something to do! Part 2. Spectrophotometry relies on what to determine DNA concentration. Reflectance of light Absorbance of any wavelength of light Absorbance of light of a specific wavelength The size of the band in a gel Part 3. What is/ are a sign of high-quality DNA when you analyze your DNA sample by gel electrophoresis? Pick all that apply. A lack of smaller bands or smear A large number of different bands One high molecular weight band A nice long smear Part 4. When the DNA sample was "specced" with the Nanovue, we got the following results: concentration 75ng.ul-1 A260/280 ratio 1.8 A260/230 ratio 2 Do the Nanovue results suggest that your DNA is free of contaminants? YES OR NO Part 5 (1st section). The original DNA sample had a concentration of 75ng.ul-1 and we need a final concentration of 10ng.ul-1 and a volume of 100ul. Using the V1C1 = V2C2 formular, calculate how many microliters (ul) (to one decimal place) of the original sample do you need to make your dilution? Part 5 (2nd section) Now that you know how much of your original DNA sample you need to make your dilution, work out how many microliters (ul) of water you will need to make your dilution up to a total volume of 100ul.
Shyam P.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD