00:01
For the first question here, the recombinant dna, these are molecules of dna from two different species and these are inserted into a host organism to produce the new genetic combinations that are important in present -day biotechnology.
00:55
And in these recombinant dna technology, here the first and initial stage, is to isolate the desired dna in its pure form.
01:13
And then in the second step, the restriction enzymes, they cut these particular dna or they cut the gene at the recognition site.
01:32
Thirdly, here the gene copies, these gene copies are amplified through the polymer's chain reaction.
01:49
And in the next step, a cut fragment of the dna and the vector.
02:01
This is joined together with the help of the enzyme dna ligase.
02:14
And at last step here the recombinant dna, this is ultimately inserted into a host.
02:29
So here we can say that the important part for the recombinant dna to make this the most useful part will be a polymerous chain reaction...