00:01
And the elastic list of reagents needed for gram staining and the order and the spin -over of each reagent in the same process.
00:20
So the first step, we will use crystal violet.
00:33
Crystal violet is a primary dye that is used in gram staining.
00:38
It's a basic dye that stains all bacteria purple, including gram positive and negative.
00:45
It's an initial staining that will stain the cell wall of the bacteria.
00:55
This is a basic dye that stains the cell wall of the bacteria purple.
01:16
This is initial stain.
01:18
The second step, we treat the sample with iodine.
01:30
Iodine would form a complex with crystal violet within the bacteria complex, and hence its retention.
01:38
Basically, when iodine is being added, it fixes the crystal violet so it cannot be washed off in gram -positive bacteria.
01:51
So the function of iodine is to form a complex with crystal violet...