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Hello students.
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The image given is a chromatography performed on the extraction of plant pigments and we have to identify the pigments extracted.
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So the first is carotene.
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Then this yellow band is xanthofil.
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This band is chlorophyll a and this band is chlorophyll b.
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Then next we have to calculate the rf value.
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Rf value is calculated by distance traveled by solute upon distance traveled by solvent.
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So what we do is we have to measure the distance traveled by the solutes and distance traveled by the solvent.
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Say suppose this is the point of origin and the salute as well as the pigments have traveled some distance.
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Say this is the distance traveled by the solvent.
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So you measure this distance.
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Then in the same manner you measure the distance traveled by carotene, xanthophyll, chlorophyll, a and quix...