00:01
All right, so here we have kind of the key parts of this peptide sequence.
00:05
These are all the amino acids that the charges are going to change based on the phs.
00:10
Whether they're on the end, that kind of the amino group and the carboxer group are very critical, or they have r groups that are impacted, like the glutamate, histine, arginine, and glycine is a carboxyl end, so that's why it's there.
00:22
So let's start off.
00:23
So with the ph that's lower than what the pca is, it'll either pronate it.
00:28
Well, it will pronate it, which either will hold in a positive charge or a neutral charge.
00:33
So, for the aminoine, of course, we're going to have a positive charge.
00:38
So in glutamate, it holds its negative charge entail it's less than 4 .3.
00:44
So it's going to be neutral because it's beneath that at 3.
00:50
And then on our histidine, it is less than 6.
00:53
So we're in a positive charge there.
00:55
Arjunine, it's less than 12 .5.
00:57
A positive charge there.
00:59
And then on that carboxal group, three is greater than 2 .3.
01:03
So we're going to have a negative charge.
01:05
So that's going to result in a 3 minus 1, a positive 2 charge at a ph of 3.
01:11
Let's put charge right there.
01:14
Just like that.
01:15
Okay, for our next one at 8, we're still in the range for the amino group is positively charged.
01:22
We're now greater than the glutamate, so that's negative.
01:25
We're greater than the histidine, so that's neutral.
01:28
So, argin, we're still in the clear for a positive.
01:31
And glycine, of course, it's still negative.
01:32
And glycine's going to be negative all the way through here...