A peptide has the sequence: Glu-His-Trp-Ser-Gly-Leu-Arg-Pro-Gly. What is the net charge of the molecule at pH 3 and 11? (Use pKa values for the side chains and terminal amino and carboxyl groups as given in table 3-1.) Estimate the pI for this protein.
Added by Cristian S.
Step 1
According to table 3-1, the pKa values are as follows: - Terminal α-carboxyl group: 2.34 - Terminal α-amino group: 9.69 - Glu (E, glutamic acid) side chain: 4.25 - His (H, histidine) side chain: 6.00 - Arg (R, arginine) side chain: 12.48 At pH 3, which is less Show more…
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