Question

A researcher inserts a human gene X, which was amplified by PCR from a human genomic library, into a bacterial plasmid. After successful transformation of bacteria, a researcher lysed the bacteria and analyzed the cell lysate by ELISA using the primary antibodies against protein X, but the protein was not detected. This might be due the fact that bacterial cells don't have a machinery for ________. Select all that apply. RNA processing post-translational modifications protein folding protein trafficking splicing The protein in its secondary structure can be found in its final destination. True False

          A researcher inserts a human gene X, which was amplified by PCR from a human genomic library, into a bacterial plasmid. After successful transformation of bacteria, a researcher lysed the bacteria and analyzed the cell lysate by ELISA using the primary antibodies against protein X, but the protein was not detected. This might be due the fact that bacterial cells don't have a machinery for ________. Select all that apply.

RNA processing
post-translational modifications
protein folding
protein trafficking
splicing

The protein in its secondary structure can be found in its final destination.

True
False

A researcher inserts a human gene X, which was amplified by PCR from a human genomic library, into a bacterial plasmid. After successful transformation of bacteria, a researcher lysed the bacteria and analyzed the cell lysate by ELISA using the primary antibodies against protein X, but the protein was not detected. This might be due the fact that bacterial cells don't have a machinery for . Select all that apply.

RNA processing
post-translational modifications
protein folding
protein trafficking
splicing

The protein in its secondary structure can be found in its final destination.

True
False

Added by William D.

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