Order the steps for the bacterial transformation procedure described in the pGlo lab Heat shock cells and immediately put on ice Incubate agar plates overnight Plate cells out on agar plates Place Bacterial Cells on ice and add pGlo plasmid Add LB broth to cells Add bacterial cells to the Calcium Chloride Solution
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Order the steps in the transformation procedure from 1 - 8 You begin with two tubes, one labeled "+" for the transformation reaction and one labeled "-" for the negative control. [ Choose ] Add 250ul transformation solution (CaCl2) to both tubes Add 250ul nutrient broth to each tube and incubate ten minutes at room temperature add DNA to the "-" tube Spread 100ul of each tube on various tester media Ice both tubes for two minutes Add DNA to the "+" tube Heat shock both tubes for 50 seconds Incubate both tubes on ice for 10 minutes Add fresh bacterial cells to both tubes Incubate both tubes on ice for 10 minutes Heat shock both tubes for 50 seconds Ice both tubes for two minutes Add 250ul nutrient broth to each tube Spread 100ul of each tube on various tester media
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