Oliver is observing his endospore slide. He sees lots of red cells, but it appears all of the endospores have been released. He needs to find a cell with an endospore still inside in order to ascertain that this species definitely has endospores. After thinking about it, Oliver remembers hearing something in lecture about the age of cells within a slant or in a colony. He then decides to repeat the stain with the current slant. What did Oliver remember about the age of cells in solid growths that will help him to find younger cells in the current slant that are more likely to have endospores inside?
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Part A: The Endospore Stain How are the differences between the endospore and the vegetative cell highlighted in the endospore staining procedure? Why must steam be used to force malachite green into the endospore? What disease would you suspect if you found endospore-forming bacteria in exudate from a patient's wound or feces? What other diseases can be diagnosed with the endospore stain? Consider the following information: The red-stained bacilli are the vegetative forms of Bacillus megaterium, and the green oval-shaped structures are endospores. Given this information, reflect on the results of your endospore stains. Did your results reflect the structures and stains described above? Why or why not?
Sri K.
You perform an endospore stain on a spore-forming bacterium, however during the staining procedure you forget to heat fix your slide and forget to steam heat your slide and instead only added the stain for 5 minutes without heat. What will the slide look like when you observe it under the microscope? Group of answer choices a- most endospore will not take up the stain because heat is required for the stain to penetrate the thick walls of the endospore. b- most endospore will still take up the stain because the staining was done for the required 5 minutes c- endospores and vegetative cells will be washed off the slide so you would see nothing on the slide because heat is required for endospores and vegetative cells to adhere to the slide. d- only some endospores will still take up the stain and will look green
Anna D.
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