On plates containing both ampicillin and arabinose, what kind of growth would you expect to see as a result of transforming E. coli cells with each of the following plasmids? (Fill in Table 2 with your predictions.) Table 2. Predictions about growth for transformation with different plasmids. 1 On plates with amp and arabinose: kanR 2 kanR pKAN-R pBAD rfp 3 ori araC pARA ampR Will colonies grow? [ Select ] [ Select ] [ Select ] Will the colonies fluoresce red under UV? [ Select ] [ Select ] [ Select ]
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ptARA: This plasmid contains the ampicillin resistance gene (ampR) and the arabinose-inducible red fluorescent protein (RFP) gene. When E. coli cells are transformed with this plasmid, they will be able to grow on plates containing ampicillin due to the presence Show more…
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You transform an E. coli bacterium with the pGLO plasmid. After growing the +pGLO bacteria on an LB+Ampicillin+Arabinose plate, you see distinct colonies form after 16 hours. You check these colonies under the UV light and see that they are glowing a bright fluorescent green color. If you use a sterile loop and inoculate a new plate with LB+Ampicillin (coated on them, as usual) with these transformed E. coli will you see any growth? If there is growth, will it glow?
Farhan A.
You transform an E. coli bacterium with the pGLO plasmid. After growing the +pGLO bacteria on an LB+Ampicillin+Arabinose plate, you see distinct colonies form after 16 hours. You check these colonies under the UV light and see that they are glowing a bright fluorescent green color. If you use a sterile loop and inoculate a new plate with LB+Ampicillin+Arabinose (coated on them, as usual) with these transformed E. coli will you see any growth? If there is growth, will it glow?A. Yes, we will see growth. Yes, they will glow. B. Yes, we will see growth. No, they will not glow. C. No, we will not see any growth. No, they will not glow. D. No, we will not see any growth. Yes, they will glow.
Dave K.
Mention in the diagram below which plates will have bacterial growth and which plates will show glowing colonies. Explain your prediction. + Plasmid - plasmid LB LB LB amp LB amp LB amp & ara LB amp & ara
Nimat P.
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