00:02
Okay, so there are a couple of jail electrophoresis questions that you need to answer.
00:09
So i'm going to start from question number one.
00:11
They're all easy questions.
00:13
So first of all, geolactrophoresis separate dnas trend based on what? so gel electrophyresis separate dna by the fragment length.
00:52
Sometimes we also call it size of the fragment.
00:58
Second question, does dna move to the positive or negative and why? dna itself carry negative charge.
01:18
And this is because the phosphate group that it carries.
01:21
So when there is an electric current flowing in the buffer, dna moves towards positive end of the electrode.
01:43
Three, western move further in the same amount of time shorter or longer.
01:50
So as i just said, gel atlachyphrates is separate dna based on the fragment length.
01:56
So longer fragment had a hard time moving through those pores in the agoros gel...