Once you had obtained a highly concentrated stock of purified phage, how would you separate the recombinant phage DNA away from the phage head and tail proteins? Just a short and concise answer
Added by Chad G.
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Step 1: Centrifuge the phage suspension to pellet the phage particles, removing any remaining cellular debris or impurities. Show more…
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Many of the routine operations in genetic engineering are carried out using commercially available "kits." Genbux Inc., a prospective manufacturer of such kits, has asked your advice on the feasibility of supplying a kit of intact $\lambda$ phage cloning vectors with the nonessential central section of their DNA already removed. Presumably a "gene jockey" could then grow the required amount of phage, isolate its DNA, and restriction cleave it without having to go to the effort of separating out the central section. What advice would you give the company?
Imagine that you are a student in Alfred Hershey and Martha Chase's lab in the late 1940 s. You are given five test tubes containing $E .$ coli bacteria infected with T2 bacteriophages that have been labeled with either $^{32} \mathrm{P}$ or $^{35} \mathrm{S}$. Unfortunately, you forget to mark the tubes and are now uncertain about which were labeled with $^{32} \mathrm{P}$ and which with $^{35} \mathrm{S}$. You place the contents of each tube in a blender and turn it on for a few seconds to shear off the phage protein coats. You then centrifuge the contents to separate the protein coats and the cells. You check for the presence of radioactivity and obtain the following results. Which tubes contained $E .$ coli infected with $^{32}$ P-labeled phage? Explain your answer. (TABLE CAN'T COPY)
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