Question

1) Once you have finished the experiment in the first video and the plates have incubated for the required time, you remove them from the incubator and document the E. coli growth in the photos below (see Figure 1 and Figure 2). Examine the agar plates within the images provided below. Record the results in a table as shown below (1 mark) pGLO plasmid added to cells? | Type of agar plate | Total # of colonies | # of green colonies under UV light No | LB | | No | LB + Ampicillin | | Yes | LB + Ampicillin | | Yes | LB + Ampicillin + Arabinose | | Figure 1. Bacterial growth of transformed and non-transformed bacteria plated on various LB agar conditions under normal lighting conditions, including (A) non-transformed bacteria on LB agar, (B) non-transformed bacteria on LB agar supplemented with ampicillin, (C) transformed bacteria on LB agar supplemented with ampicillin, and (D) transformed bacteria on LB agar supplemented with both ampicillin and arabinose.

          1) Once you have finished the experiment in the first video and the plates have incubated for the required time, you remove them from the incubator and document the E. coli growth in the photos below (see Figure 1 and Figure 2). Examine the agar plates within the images provided below. Record the results in a table as shown below (1 mark)

pGLO plasmid added to cells? | Type of agar plate | Total # of colonies | # of green colonies under UV light
No | LB | | 
No | LB + Ampicillin | | 
Yes | LB + Ampicillin | | 
Yes | LB + Ampicillin + Arabinose | | 

Figure 1. Bacterial growth of transformed and non-transformed bacteria plated on various LB agar conditions under normal lighting conditions, including (A) non-transformed bacteria on LB agar, (B) non-transformed bacteria on LB agar supplemented with ampicillin, (C) transformed bacteria on LB agar supplemented with ampicillin, and (D) transformed bacteria on LB agar supplemented with both ampicillin and arabinose.

1) Once you have finished the experiment in the first video and the plates have incubated for the required time, you remove them from the incubator and document the E. coli growth in the photos below (see Figure 1 and Figure 2). Examine the agar plates within the images provided below. Record the results in a table as shown below (1 mark)

pGLO plasmid added to cells? | Type of agar plate | Total # of colonies | # of green colonies under UV light
No | LB | |
No | LB + Ampicillin | |
Yes | LB + Ampicillin | |
Yes | LB + Ampicillin + Arabinose | |

Figure 1. Bacterial growth of transformed and non-transformed bacteria plated on various LB agar conditions under normal lighting conditions, including (A) non-transformed bacteria on LB agar, (B) non-transformed bacteria on LB agar supplemented with ampicillin, (C) transformed bacteria on LB agar supplemented with ampicillin, and (D) transformed bacteria on LB agar supplemented with both ampicillin and arabinose.

Added by Anna S.

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