Oxygen consumption by isolated mitochondria can be measured in a device called an oxygen electrode. Typical data are shown below, where the slope of the trace is a measure of the rate of oxygen consumption. In the experiment shown below, mitochondria were provided with a respiratory substrate (NADH) and inorganic phosphate. Explain the response of the mitochondria to the successive additions of ADP, oligomycin (an inhibitor of ATP synthase) and dinitrophenol.
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Step 1: Mitochondria are provided with respiratory substrate (NADH) and inorganic phosphate in the experiment. Show more…
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