p-nitrophenylphosphate pNPP (colorless) HO Phosphate P nitrophenol o nitrophenyl phosphate (pNPP) Ao Alkaline condensation NaOH promotes the formation of the phenolate ion and intensification of color p-nitrophenolate ion pNP (colored) trinitrophenolate T gbiosciences com pNP absorbs light at 405 nm, ε = 18000 M^-1 cm^-1
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The Beer-Lambert Law states that: $A = \epsilon \times l \times c$ Where: $A$ is the absorbance $\epsilon$ is the molar absorptivity (18000 M$^{-1}$ cm$^{-1}$) $l$ is the path length (1 cm) $c$ is the concentration of the solution (in M) Show more…
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