Part C What conclusion would you draw if some of the proteins that become labeled by the LP/125I method of part B were among those that had been labeled when intact cells were incubated with LP in the presence of 125I and the membrane proteins, then extracted and analyzed on SDS – polyacrylamide gels? Even if these proteins are settle only from one side of the membrane, because of their large charge they can attract labels from the other side. Some proteins extend all the way through the membrane and actually protrude sufficiently on both sides of the membrane to allow them to be labeled on either side. Even though some proteins protrude only on one side of the membrane, after labeling they change their conformation that allows them to penetrate to the other side of the membrane and to be labeled.
Added by Keith A.
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Step 1: Identify the proteins labeled by the LP/125I method in both intact cells and membrane proteins extracted and analyzed on SDS-polyacrylamide gels. Show more…
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