You transformed bacteria with varies plasmids and counted the colonies as well as colony color. All bacteria were plated on media containing ampicillin. See below. Transformed with Colonies observed 1. Plasmid with GFP gene 100 green colonies 2. Plasmid without GFP gene 100 white colonies 3. No plasmid No colonies What conclusions are supported by these results? Identify the results that support each claim. GFP can be expressed from the plasmid colony growth from #1 and #: The bacteria are naturally ampicillin resistant Claim not supported The plasmid contains a gene for ampicillin resistance No colonies growing on #3
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There is transformed bacteria with varied plasmids, counting the colonies as well as the colony color. All the bacteria were plated on media containing ampicillin. See below Transformed with: Colonies Observed 1. Plasmid with GFP Gene: 200 green colonies 2. Plasmid without GFP Gene: 200 white colonies 3. No Plasmid: No colonies What conclusions are supported by these results. Pick an option out of the four given that supports each claim below. The choices are below to be applied for each claim. A. Claim not supported B. 200 green colonies from #1 C. Colony growth from #1 and #2 but not #3 D. No colonies growing on #3 First Claim: GFP can be expressed from the Plasmid, What's the correct option? Second Claim: The plasmid contains a gene for ampicillin resistance, What's the correct option? Third Claim: The bacteria are naturally ampicillin resistant, What's the correct option?
Josee P.
a. What colony phenotypes (traits) allowed you to confirm that the bacteria were transformed by the pGLO plasmid? b. Which two of the four plates best demonstrates the role of arabinose as a gene regulator for the expression of the GFP gene? Explain your answer. c. Which plate(s) would you inspect to conclude that transformation occurred successfully, i.e. cells acquired a complete plasmid containing both gene markers? Explain why. e. Why do you think the number of transformed colonies on the transformation plates containing ampicillin varied between lab groups? What factors may have affected transformation efficiency, i.e. the number of colonies produced per mass of plasmid used? f. If an antibiotic other than ampicillin was used in the selective growth media, do you think the results would be same or different for bacteria transformed with the pGLO plasmid? To help you explain your answer, locate a resource that describes how the different modes of action of different antibiotics function to impede bacterial growth.
Suman K.
Let's suppose you followed the protocols described in Figures 20.2 and $20.3 .$ Which experiment(s) would you conduct to confirm that a white colony really contained a recombinant vector with an inserted fragment of DNA? a. Pick a bacterial colony and restreak on plates containing $\mathrm{X}$ -Gal to confirm that the cells really form white colonies. b. Pick a bacterial colony, isolate plasmid DNA, digest the plasmid DNA with a restriction enzyme, and then perform gel electrophoresis with the DNA. c. Pick a bacterial colony and test it to see if $\beta$ -galactosidase is functional within the bacterial cells. d. Pick a bacterial colony and retest it on ampicillin-containing plates to double-check that the cells are really ampicillin resistant. e. Both c and d should be conducted.
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