Question

PCR Primers: (I) 5'GCCTACTT3' and (II) 5'-CCGGATCC-3' 5'-AGGCCTGCTCGCCTACTTAAGGGCCTACGATGCAGCCTGGATCCGGATCG-3' a) Which primer (I) or (II) would bind to the above strand? b) Where would the primer bind, please underline the 8 bases? c) You now add DNA polymerase (Remembering DNA polymerases produce new DNA 5' to 3'), dNTPs, and MgCl2. What would the sequence of the new strand of DNA look like (include primer)? 5'- d) You now denature the double stranded DNA. e) Which primer (I) or (II) would bind to the new strand in answer c? f) Where would the primer bind to the new strand in answer c, please underline the 8 bases? g) You now add DNA polymerase (Remembering DNA polymerases produce new DNA 5' to 3'), dNTPs, and MgCl2. What would the sequence of the resulting strand of DNA look like? 5'- With multiple rounds almost all the DNA will become the resulting strand.

          PCR
Primers:
(I) 5'GCCTACTT3' and (II) 5'-CCGGATCC-3'
5'-AGGCCTGCTCGCCTACTTAAGGGCCTACGATGCAGCCTGGATCCGGATCG-3'
a) Which primer (I) or (II) would bind to the above strand?
b) Where would the primer bind, please underline the 8 bases?
c) You now add DNA polymerase (Remembering DNA polymerases produce new DNA 5' to 3'), dNTPs, and MgCl2. What would the sequence of the new strand of DNA look like (include primer)?
5'-
d) You now denature the double stranded DNA.
e) Which primer (I) or (II) would bind to the new strand in answer c?
f) Where would the primer bind to the new strand in answer c, please underline the 8 bases?
g) You now add DNA polymerase (Remembering DNA polymerases produce new DNA 5' to 3'), dNTPs, and MgCl2. What would the sequence of the resulting strand of DNA look like?
5'-
With multiple rounds almost all the DNA will become the resulting strand.

Show more…

PCR
Primers:
(I) 5'GCCTACTT3' and (II) 5'-CCGGATCC-3'
5'-AGGCCTGCTCGCCTACTTAAGGGCCTACGATGCAGCCTGGATCCGGATCG-3'
a) Which primer (I) or (II) would bind to the above strand?
b) Where would the primer bind, please underline the 8 bases?
c) You now add DNA polymerase (Remembering DNA polymerases produce new DNA 5' to 3'), dNTPs, and MgCl2. What would the sequence of the new strand of DNA look like (include primer)?
5'-
d) You now denature the double stranded DNA.
e) Which primer (I) or (II) would bind to the new strand in answer c?
f) Where would the primer bind to the new strand in answer c, please underline the 8 bases?
g) You now add DNA polymerase (Remembering DNA polymerases produce new DNA 5' to 3'), dNTPs, and MgCl2. What would the sequence of the resulting strand of DNA look like?
5'-
With multiple rounds almost all the DNA will become the resulting strand.

Added by Jordan F.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

Thanks for your feedback!

PCR Primers: (I) 5'GCCTACTT3' and (II) 5'-CCGGATCC-3' 5'-AGGCCTGCTCGCCTACTTAAGGGCCTACGATGCAGCCTGGATCCGGATCG-3' a) Which primer (I) or (II) would bind to the above strand? b) Where would the primer bind, please underline the 8 bases? c) You now add DNA polymerase (Remembering DNA polymerases produce new DNA 5' to 3'), dNTPs, and MgCl2. What would the sequence of the new strand of DNA look like (include primer)? 5'- d) You now denature the double stranded DNA. e) Which primer (I) or (II) would bind to the new strand in answer c? f) Where would the primer bind to the new strand in answer c, please underline the 8 bases? g) You now add DNA polymerase (Remembering DNA polymerases produce new DNA 5' to 3'), dNTPs, and MgCl2. What would the sequence of the resulting strand of DNA look like? 5'- With multiple rounds almost all the DNA will become the resulting strand.

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Transcript

00:01 You have two primers, one and two, so i list a primer on the top part of the page.

00:08 Now, you're also given a dna sequence, so i have written one of the strength.

00:13 Question number eight, which primer one or two would bind to the above strength? so in this case, it's going to be number two primer.

00:23 So number two primer is going to bind somewhere here.

00:31 C, c, g, g, a, t, c, c.

00:45 So you can see the primer is complementary to the template dna strength, follow a pair up with t, c pair up with g.

00:57 And the primer and the template string, they have opposite orientation.

01:03 So we can see for the template dna 3 priming is on the right side, but the primer has 5 primate on the right side.

01:14 Where would the primer bind please under 9 8 basis? so as you can see that obviously it match up with these 8, 1, 2, 3, 4, 5, 6, 7, 8.

01:28 Now, you now add dna polymerase, dntps, and magnesium chloride.

01:34 What will be the sequence of the new gene of dna look like, including primers? so you can see that the red part is the primer.

01:42 And then this is the three primate.

01:48 So basically dna polymerase is going to add nucleotide to the three primate of the primer and extend from right to left direction.

01:57 And so i'm going to use the green color to write down the rest of the sequence.

02:05 So it is going to extend from right to left.

02:09 So what we have here is the last c right here.

02:13 This is primer.

02:15 So the next dntp is going to be a, pair up with c.

02:22 So you can see i'm continue to write down the sequence according to the base pair of rule.

02:29 A pair with t, c pair up with g.

03:26 Okay, so now i write down the bottom strand is green.

03:31 So this is going to be the new strand that made by the primer, red primer.

03:40 All right...

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