Perform 5-fold serial dilution: Stock 1 2 3 4 5 6 7 0.2 ml 0.2 ml 6.2 ml 0.2 ml 0.2 ml 0.2 ml 0.2 ml (10 What is the concentration of tube 7? Show your work. mM)
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Calculate the concentration of cells in the flask using cell concentration data provided for tubes 2, 3, and 4. Hint: Use the appropriate dilution factor. Show your work. (1pt) Cell Concentration in each tube | Total Dilution Factor | Cell Concentration in the flask Tube #2: 9.84x10^7 cells/ml | 1:2 | 1/2(9.84x10^7) = Tube #3: 4.92x10^7 cells/ml | 1:2 | Tube #4: 2.46x10^7 cells/ml | 1:2 | Does it appear you have a good serial dilution of the cells? Explain. (1pt)
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In lab, you will use a stock solution that is 2 mM of KMnO4 in a serial dilution to make 6 mL of each of the following concentrations: 1.0 mM, 0.5 mM, 250 µM, 125 µM, 62.5 µM, 31.3 µM, 15.6 µM. Remember to include the units in your answers, where appropriate! 1. What is the dilution factor for this serial dilution? 2. What is V2 for this serial dilution? 3. Calculate V1 for this serial dilution. Show your calculations. 4. What volume of water will you add to each tube?
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Calculate the concentration of cells in the flask using cell concentration data provided tubes 2, 3, and 4. Hint: Use the appropriate dilution factor. Show your work. Tube #2: Cell Concentration in each tube: 9.84x10^7 cells/ml Tube #3: Cell Concentration in each tube: 4.92x10^7 cells/ml Tube #4: Cell Concentration in each tube: 2.46x10^7 cells/ml Does it appear you have a good serial dilution of the cells? Explain.
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