Question

Performing a bacterial isolation by streaking, using sterile (aseptic) technique, consider the expected outcome on the streak plate after the following: Two organisms were in the original sample. Organism G was present at twice the abundance of Organism H (so 2/3 of the original sample). The streak plate was generated using 4 areas (quadrants) – A, B, C & D. Area A was inoculated with a loop containing 3 million organisms from the original culture, which was mixed very well and considered to be homogeneous. Areas B, C & D were streaked from the previous area with a sterilized loop, with each streak transferring 2% of the organisms from the previous area (assume the relative abundance of the two organisms was maintained). What is the expected outcome in Area D on this streak plate? (4 pts)

          Performing a bacterial isolation by streaking, using sterile (aseptic) technique, consider the expected outcome on the streak plate after the following:
Two organisms were in the original sample. Organism G was present at twice the abundance of Organism H (so 2/3 of the original sample).
The streak plate was generated using 4 areas (quadrants) – A, B, C & D. Area A was inoculated with a loop containing 3 million organisms from the original culture, which was mixed very well and considered to be homogeneous. Areas B, C & D were streaked from the previous area with a sterilized loop, with each streak transferring 2% of the organisms from the previous area (assume the relative abundance of the two organisms was maintained).
What is the expected outcome in Area D on this streak plate? (4 pts)

Performing a bacterial isolation by streaking, using sterile (aseptic) technique, consider the expected outcome on the streak plate after the following:
Two organisms were in the original sample. Organism G was present at twice the abundance of Organism H (so 2/3 of the original sample).
The streak plate was generated using 4 areas (quadrants) – A, B, C D. Area A was inoculated with a loop containing 3 million organisms from the original culture, which was mixed very well and considered to be homogeneous. Areas B, C D were streaked from the previous area with a sterilized loop, with each streak transferring 2% of the organisms from the previous area (assume the relative abundance of the two organisms was maintained).
What is the expected outcome in Area D on this streak plate? (4 pts)

Added by Michael R.

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