00:01
Dilution chart with specimen tube was one or two dilution, one or three, one or four, one or five, one or six.
00:06
So the question asks you to decide the volume of the standard and the volume of the diluent.
00:13
Remember, you need two mil total for each dilution.
00:16
Ok, so let's start with the first one or two dilution.
00:23
So you will need one milliliter of the sample, or we say the standard sample.
00:35
And the total volume is going to be one plus one, which is two.
00:45
So you will have one mil of standard sample and then one mil of distilled water.
00:51
So it's a one to two dilution.
00:54
The volume increase two times, the concentration drops two times.
00:58
Now let's calculate the final absorbance.
01:02
The final absorbance is going to be the one that you have, 0 .179 times dilution factor, which is two.
01:13
So you get 0 .358.
01:19
The next one is 1 third.
01:28
This we can actually use this formula, c1 times v1 equals c2 times v2.
01:35
C1 is the original concentration.
01:37
Let's say 1.
01:39
V1 is 1x.
01:43
V1 is the volume they take from the 1x.
01:47
C2 is 1 third, because the c2 is 1 third of the original 1x.
01:53
So 1 third x.
01:56
And v2 we know is 2 milliliter.
01:59
So v1 is going to a third of 2.
02:04
So basically, 2 out of 3 mil divided by 1.
02:10
You need 0 .67 milliliter.
02:17
So you need 0 .67 standard sample.
02:22
And the diluent that you need, or water, is going to be 2 mil minus 0 .67.
02:31
This equals 1 .33 milliliter.
02:34
So this goes into the 1 to 3 dilution.
02:37
You need 0 .67 mil of standard sample with 1 .33 milliliter of diluent.
02:44
And the final absorbance can be calculated by the third column, 0 .118 times the dilution factor here, which is 3.
03:00
You get 0 .354.
03:04
So the dilution factor is.
03:11
Now the next one is a 1 to 4 dilution.
03:17
So we are going to use the same formula...