Question

Plate | Number of colonies: LB, no DNA | uncountable LB/ amp, no DNA | 0 LB/ amp, + DNA (The two plated labeled Bacteria + DNA) | 683 (70 ?l) 291 (30 ?l) (a) What quantity of DNA (in ng) was used in the transformation? (2 marks) (b) What fraction of the total transformation reaction was plated out? (2 marks) (c) How many transformants (colonies) were there in the total transformation reaction? (2 marks) (d) What was the transformation efficiency, expressed as transformants per ?g of DNA added? (3 marks) If the highest efficiency obtainable by an optimal method is around 1 x 10^8 transformants per ?g and a poor efficiency is around 1 x 10^3, how does your transformation efficiency compare? (1 mark)

          Plate | Number of colonies:
LB, no DNA | uncountable
LB/ amp, no DNA | 0
LB/ amp, + DNA (The two plated labeled Bacteria + DNA) | 683 (70 ?l)
291 (30 ?l)
(a) What quantity of DNA (in ng) was used in the transformation? (2 marks)
(b) What fraction of the total transformation reaction was plated out? (2 marks)
(c) How many transformants (colonies) were there in the total transformation reaction? (2 marks)
(d) What was the transformation efficiency, expressed as transformants per ?g of DNA added? (3 marks)
If the highest efficiency obtainable by an optimal method is around 1 x 10^8 transformants per ?g and a poor efficiency is around 1 x 10^3, how does your transformation efficiency compare? (1 mark)

$Plate | Number of colonies: LB, no DNA | uncountable LB/ amp, no DNA | 0 LB/ amp, + DNA (The two plated labeled Bacteria + DNA) | 683 (70 ?l) 291 (30 ?l) (a) What quantity of DNA (in ng) was used in the transformation? (2 marks) (b) What fraction of the total transformation reaction was plated out? (2 marks) (c) How many transformants (colonies) were there in the total transformation reaction? (2 marks) (d) What was the transformation efficiency, expressed as transformants per ?g of DNA added? (3 marks) If the highest efficiency obtainable by an optimal method is around 1 x 10^8 transformants per ?g and a poor efficiency is around 1 x 10^3, how does your transformation efficiency compare? (1 mark)$

Added by Alvin W.

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