please explain in in depth details in microbiology of how do gene expression get regulated what

Please explain in in depth details in microbiology of how do...

Transcript

00:02 The negative negative gene regulation gene regulation so in the negative gene regulation what happens it occurs it occurs when a regulatory protein when a regulatory protein a regulatory protein that is called as a repressor called as repressor when this regulatory protein binds with the operator region binds with operator region operator region of lac operon lac operon negative gene regulation after that it prevents prevents prevents the process of transcription of the genes.

01:22 In the case of lac operon, this negative gene regulation this negative gene regulation is mediated by is mediated by a repressor protein called a repressor protein which is called as lac1 protein one protein now let us understand the effect of this lag one protein lag one protein how does this impact this protein is produced this protein is produced constitutively by a scar isis -coli constitutively by a scar isis -coli meaning it is synthesized means it is synthesized continuously regardless lactose is present or not.

03:31 Regardless the lactose is present or not after that in the operator region what happens this the operator region we know the operator region is a specific it is a specific dna sequence specific dna sequence within the lac operon within the lac operon to which this lsc1 repressor protein binds prevents transcription prevents transcription now there is two case scenario number one when lactose van in a medium lactose is absent so in this case what happens in the absence of lactose the lac1 repressor the lac1 repressor protein binds tightly with binds tightly to the operator region to operator region and inhibits the transcription this inhibits transcription chance encryption now this is the default state this is actually a default statement lactose is absent this is a default state where x -rayces -coli that is the e -coli is does not waste energy does not waste energy does not waste energy producing the unnecessary producing unnecessary lactose metabolizing lactose metabolizing metabolizing enzymes and this happens when the lactose is absent.

06:36 On the other case when the lactose is present, when lactose is present, in this case the lactose, a fraction of a lactose a fraction of lactose molecules got converted into some of allolectose it converts into allolectose which acts as an inducer molecule this act as inducer molecule allolectase then binds with this allolectose and then binds with lat1 repressor protein repressor that causes the conformational changes that causes conformational changes conformational changes in the repressor protein in repressor protein talking about the positive gene regulation the positive gene regulation so what happens in positive gene regulation this action involves the action of a action of a regulatory protein a regulatory protein called as called as activator all as active later this activator enhances the transcription enhances the transcription by binding with by binding with specific dna sequence with the specific dna dna sequence.

09:14 In the case of lac operon, in the case of lac operon, the positive gene regulation, positive gene regulation is mediated by, is mediated by, mediated by the campcrp complex.

09:45 Complex...

Question

please explain in in-depth details in microbiology of how do gene expression get regulated? what systems involved in gene expression regulation? please give great detailed examples and real life examples?

Question

please explain in in-depth details in microbiology of how do gene expression get regulated? what systems involved in gene expression regulation? please give great detailed examples and real life examples?

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