00:01
The first question is asking about the disadvantages of producing recombinant proteins in bacteria, east as well as the plant cells.
00:19
First is bacterial cell.
00:23
In bacterial cells it may happen that some recombinant proteins gets accumulated as inclusion bodies and it occurs because of their overproduction.
00:36
The second point is host protease may degrade the expressed protein.
00:43
The next is humans or mammalian needs.
00:44
Genes flown in the bacterial cells cannot undergo splicing event.
00:50
It is because the bacterial cells lack the splicing machinery.
00:56
From the bacterial cells it is difficult to obtain a multi -domain protein, for example antibodies because the bacterial cells do not allow the correct or proper folding of the proteins as well as the bacterial cells also lacks post -translational modifications.
01:23
Second is east cell and some of the challenges are.
01:29
Associated with yeast cells includes in east cell there observed hyperglycosylation by both n and o linked oligosaccharides on the expressed protein that is the recombinant protein.
01:49
The east cells also lack strong promoters...