You extracted a sample of DNA and analyzed it using a Nanodrop spectrophotometer. You recorded the following absorbances and then corrected them to produce the following values: A260 = 0.51 A280 = 0.66 Calculate the quality of DNA in the sample using these corrected absorbance(s). Report your answer to two decimal places (e.g., 1.00, 1.11, etc.) and DO NOT include units. Your Answer: Answer Question 4 (2 points) In this space, show your work for the question above. Be sure that your work: • provides the A260 value • provides the A280 value • identifies the equation that you used to solve the problem, • identifies which numbers you substituted for which variables in the equation, • exhibits all the necessary steps needed to show the math used to solve this problem and provide an answer
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Obtain absorbance values for DNA sample using Nanodrop spectrophotometer. Show more…
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You are a researcher measuring the concentration of DNA in a solution using a spectrophotometer. You determine that the absorbance of a solution (measured at 260 nm) is 1.7 If you used a 1 cm cuvette and the extinction coefficient of DNA is 0.020 (<μg/ml/cm), what is the concentration of the DNA in μg/ml? (DO NOT INCLUDE UNITS IN YOUR ANSWER BELOW) Answer:
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Text: DNA quantification by spectrophotometer Q1. Principle of DNA quantification by spectrophotometer? Q2. What will be the result of DNA quantification by spectrophotometer if the cuvette is inserted with the opaque side in the spectrophotometer? Q3. At which wavelength does DNA show absorbance? Q4. What is optical density (O.D) and its relationship with the amount (concentration) of DNA to be determined? Q5. O.D of blank? Q6. How is the purity of a DNA sample determined (what is the A260:A280 ratio)? Q7. For a pure DNA sample, what should be the value of the A260:A280 ratio? Q8. What do the peaks at A230 and A280 correspond to in the given testing sample? Q9. What will be the O.D of the given sample (1ug/ml) at A260 and cuvette size (1 cm)? Q10. Problem: 1 ug DNA = 0.02 O.D; A260 If the dilution factor (DF) = 50, what will be its O.D and DNA amount present? Calculate the amount of DNA (x ug if O.D = 0.123, A260, unknown DNA = 1 ul, DF = 1:15
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