Please place the statements regarding CRISPR function into the correct order. Rank the options below. Cas proteins cut entering viral DNA into short fragments. The bacterial cell transcribes the CRISPR array. crRNAs are generated by the bacterial cell. Viral DNA fragments are inserted into a CRISPR array. crRNA binds a Cas nuclease, forming a complex.
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Place the following components of CRISPR-Cas systems in the order in which they function during generation of CRISPR-Cas immunity. a. Effector complex binds to the foreign DNA b. Transcription of CRISPR array into a long CRISPR precursor RNA c. crRNA combines with a Cas protein to form an effector complex d. Incorporation of spacers into CRISPR array e. Cas protein in the effector complex cleaves the foreign DNA f. CRISPR precursor RNA (pre-crRNA) is cleaved and processed into crRNAs
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Which of the following is found in a crRNA (i.e., the processed CRISPR RNA) that is ready to be used as a guide RNA to identify the target DNA or RNA? a) One full and part of another repeat sequence and one complete spacer sequence. b) One PAM sequence, one full repeat sequence, and one full spacer sequence. c) Two full repeat sequences and two complete spacer sequences. d) The leader sequence and two spacer sequences flanking each of the repeat sequences. How do the 6S RNAs affect gene expression in E. coli cells growing at the stationary phase of growth? A) 6S RNAs bind Sigma S-RNAP complexes and help transcribe genes supposed to be activated during the stationary phase. B) 6S RNAs bind Sigma 70-RNAP holoenzyme complex and break up/degrade the holoenzyme. c) 6S RNAs bind Sigma 70-RNAP complexes and prevent Sigma 70-RNAP from binding gene promoters. All of the following are functions of the cas proteins (or the cascade complex) in the CRISPR phenomenon of E. coli, EXCEPT a) processing the primary CRISPR RNA b) identifying and slicing the viral DNA or viral RNA. c) acquiring the spacer DNA from virus genomes d) transcribing the primary CRISPR RNA locus
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