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7) Imagine that you are a biomedical engineer in cell culture laboratory of a company. You are working project about drug discovery against a Covid-19. You want to test efficacy of 10 nM, 100 nM, 500 nM and 1000 nM dosages of a drug as triple. Then, you will compare those dosage with control group (0 nM). You will perform the study in 24 well plate. Each well of 24 well plate should include $5 \times 10^5$ cells. In light of this informations, you used hemocytometer to seed cells to wells and your calculations were from 10 ml cell suspension as given in below: Number of Live Cells : 297 Number of Death Cells : 3 Trypan Blue : 20 ?l Cell Suspension from 10 ml: 20 µl PBS : 960 ?l Number of Counted Squares: 10 a) Draw your toxicity determination process of drug dosages groups against Covid-19 in 24 well plate in Table. (5 points) Name: Student Number: December 3, 2020 b) Briefly, explain why do you need to dilution? If you didn't use any dilution factor, how would you affect your experiment? (5 pts) c) Calculate how much µl could you take from cell suspension to obtain $5 \times 10^5$ cells in a well? (10 points)

          7) Imagine that you are a biomedical engineer in cell culture laboratory of a company. You are
working project about drug discovery against a Covid-19. You want to test efficacy of 10 nM, 100
nM, 500 nM and 1000 nM dosages of a drug as triple. Then, you will compare those dosage with
control group (0 nM). You will perform the study in 24 well plate. Each well of 24 well plate should
include $5 \times 10^5$ cells. In light of this informations, you used hemocytometer to seed cells to wells
and your calculations were from 10 ml cell suspension as given in below:
Number of Live Cells
: 297
Number of Death Cells
: 3
Trypan Blue
: 20 ?l
Cell Suspension from 10 ml: 20 µl
PBS
: 960 ?l
Number of Counted Squares: 10
a) Draw your toxicity determination process of drug dosages groups against Covid-19 in 24 well
plate in Table. (5 points)
Name:
Student Number:
December 3, 2020
b) Briefly, explain why do you need to dilution? If you didn't use any dilution factor, how would you
affect your experiment? (5 pts)
c) Calculate how much µl could you take from cell suspension to obtain $5 \times 10^5$ cells in a well? (10
points)

7) Imagine that you are a biomedical engineer in cell culture laboratory of a company. You are
working project about drug discovery against a Covid-19. You want to test efficacy of 10 nM, 100
nM, 500 nM and 1000 nM dosages of a drug as triple. Then, you will compare those dosage with
control group (0 nM). You will perform the study in 24 well plate. Each well of 24 well plate should
include 5 × 10^5 cells. In light of this informations, you used hemocytometer to seed cells to wells
and your calculations were from 10 ml cell suspension as given in below:
Number of Live Cells
: 297
Number of Death Cells
: 3
Trypan Blue
: 20 ?l
Cell Suspension from 10 ml: 20 µl
PBS
: 960 ?l
Number of Counted Squares: 10
a) Draw your toxicity determination process of drug dosages groups against Covid-19 in 24 well
plate in Table. (5 points)
Name:
Student Number:
December 3, 2020
b) Briefly, explain why do you need to dilution? If you didn't use any dilution factor, how would you
affect your experiment? (5 pts)
c) Calculate how much µl could you take from cell suspension to obtain 5 × 10^5 cells in a well? (10
points)

Added by Michael M.

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