Polymerase chain reaction (PCR) is a powerful tool in molecular biology that is used to amplify a particular DNA sequence. If a PCR reaction initially contains 1 double-stranded DNA copy of the sequence of interest, how many copies of double-stranded DNA will be generated after 11 cycles? Assume perfect doubling occurs in each cycle?
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Step 1: In PCR, the number of double-stranded DNA copies doubles in each cycle. Show more…
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Polymerase chain reaction (PCR) is a technique used to amplify (copy) DNA. Suppose a single, linear molecule of double-stranded DNA (dsDNA) is amplified by PCR. After one PCR cycle, how many molecules of dsDNA will there be?
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If each cycle of $\mathrm{PCR}$ doubles the amount of DNA synthesized in the previous cycle, then 10 cycles will give a $10^{3}-$ fold amplification, 20 cycles will give a $10^{6}$ -fold amplification, and 30 cycles will give a $10^{9}$ -fold amplification.
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