Predict what Crick and Brenner would have found if codons were two bases long. Select the two correct statements. One-base-pair insertions or deletions would disrupt protein function because of changing the reading frame. One-base-pair insertions or deletions would NOT disrupt protein function because the reading frame would be maintained. Both one-base-pair and adjacent two-base-pair insertions or deletions would destroy protein function because of changing the reading frame. Adjacent two-base-pair insertions or deletions would NOT destroy protein function because the reading frame would be maintained. Adjacent two-base-pair insertions or deletions would destroy protein function because of changing the reading frame.
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What would be the effect on reading frame and gene function if: Two bases were inserted into the middle of an mRNA? Three bases were inserted into the middle of an mRNA? One base was inserted into one codon? Two codons were subtracted from the first codon?
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Part A Assuming the genetic code is a triplet, what effect would the addition or loss of two nucleotides have on the reading frame? It will have no effect on the reading frame. It will have no effect on the reading frame, but will result in the addition or loss of amino acids in the resulting protein. It will change the reading frame. It will change the reading frame and result in the addition or loss of amino acids in the resulting protein. Part B What effect would the addition or loss of three, six, or nine nucleotides have on the reading frame? It will change the reading frame. It will change the reading frame and result in the addition or loss of amino acids in the resulting protein. It will have no effect on the reading frame, but will result in the addition or loss of amino acids in the resulting protein. It will have no effect on the reading frame.
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Which of the following describes the most likely effect that a single base substitution in the middle of a gene's sequence will have on the protein the gene encodes? There would be no change because the genetic code is ambiguous. A single amino acid would change because pairing of mRNA and tRNA in the ribosome would change. All amino acids following the substitution would be affected because the reading frame would be altered. The protein would be shorter because the correct tRNA would not be able to interact with the ribosome.
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