Predict whether ?-galactosidase (B-gal) will be produced by the following E. coli strains under the conditions noted. The diploid genotypes represent F' (lac) strains. For each strain, use a plus sign (+) to indicate that B-gal is synthesized, and a minus sign (0) to indicate that the B-gal is not synthesized in both the absence and presence of lactose (list your answers in like this example - #11 0 and +). In this scenario, glucose is NOT present in the medium. Genetic Strain of E. coli Bacteria | Lactose Absent | Lactose Present 1. I+ P+ O+ Z+ 2. IS P+ O+ Z+ 3. I+ P+ OC Z+ 4. I- P+ OC Z+ 5. IS P+ O+ Z+/I+ P+ OC Z+ 6. I+ P+ OC Z-/I- P+ O+ Z+ 7. I+ P+ O+Z-/I- P+ O+ Z+ 8. I- P+ O+ Z-/I- P+ OC Z- 9. I+ P+ OC Z-/I- P+ O+ Z+ 10. IS P+ OC Z-/I+ P+ O+ Z+
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coli bacteria for each scenario: - I*P* 0*2' IS P+0*2* - I*P* 0c 2* TPFocz TSP*0 - Z71*P*ocz' TPocz/P*0*z' - rpoziP*0*z IPro z/ P*0cz - TPocz/lp*0*z' TP-oczmP-oz' Show more…
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Analyze the following genotypes of the Lac Operon in E. coli. In each case, determine whether Beta-galactosidase (enzyme that digests lactose) and permease (membrane channel that brings lactose into the cell) would be made in the presence or absence of lactose. Simply select 'YES' if the enzyme is made or 'NO' if it is not. (Refer to the Lac Operon diagram for the meaning of the letters in the genotypes. Remember a "plus" superscript indicates normal functioning part of operon or repressor gene, a "minus" indicates loss of function, "OC" means "operator-constitutive", and "IS" means "super-repressor")
Sri K.
Madhur L.
Groups of alleles associated with the lactose operon are as follows (in order of dominance for each allelic series): repressor, $I^{s}$ (superrepressor), $I^{+}$ (inducible), and $I^{-}(\text {constitutive }) ;$ operator, $O^{c}$ (constitutive, $c i s^{-}$ dominant) and $O^{+}$ (inducible, cis-dominant); structural, $Z^{+}$ and $Y^{+}$ (a) Which of the following genotypes will produce (a) Which of the following genotypes will produce \beta-galactosidase and $\beta$ -galactoside permease if lactose is present: (1) $I^{+} O^{+} Z^{+} Y^{+},$ (2) $I^{-} O^{c} Z^{+} Y^{+},$ (3) $I^{s} O^{c} Z^{+} Y^{+}$ (4) $I^{s} O^{+} Z^{+} Y^{+}$, and (5)$I^{-} O^{+} Z^{+} Y^{+}$ ? (b) Which of the above genotypes will produce $\beta$ -galactosidase and $\beta$ -galactoside permease if lactose is absent? Why?
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