Question

PRELAB ASSIGNMENT - LABORATORY 9 1. Draw a flowchart or brief listing of the laboratory procedure that you will follow to extract and purify GFP from bacterial cells. 2. Green Fluorescent Protein has regions that are very hydrophobic. What is the meaning of "hydrophobic"? What causes a protein to be hydrophobic? 3. In your own words, describe how we will use hydrophobic interaction chromatography to purify GFP from the multitude of other proteins in a bacterial cell. Be sure to include the purpose of each of the wash solutions in the process (Equilibration, Binding, Wash, and Elution Buffers).

          PRELAB ASSIGNMENT - LABORATORY 9
1. Draw a flowchart or brief listing of the laboratory procedure that you will follow to extract
and purify GFP from bacterial cells.
2. Green Fluorescent Protein has regions that are very hydrophobic. What is the meaning of
"hydrophobic"? What causes a protein to be hydrophobic?
3. In your own words, describe how we will use hydrophobic interaction chromatography to
purify GFP from the multitude of other proteins in a bacterial cell. Be sure to include the
purpose of each of the wash solutions in the process (Equilibration, Binding, Wash, and Elution
Buffers).

PRELAB ASSIGNMENT - LABORATORY 9
1. Draw a flowchart or brief listing of the laboratory procedure that you will follow to extract
and purify GFP from bacterial cells.
2. Green Fluorescent Protein has regions that are very hydrophobic. What is the meaning of
"hydrophobic"? What causes a protein to be hydrophobic?
3. In your own words, describe how we will use hydrophobic interaction chromatography to
purify GFP from the multitude of other proteins in a bacterial cell. Be sure to include the
purpose of each of the wash solutions in the process (Equilibration, Binding, Wash, and Elution
Buffers).

Added by Michael W.

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