Question

Prokaryotic cells have 3 DNA polymerases: DNA Pol I, II, and III. How are these similar to each other and how are they different? Eukaryotic cells have 4 DNA polymerases involved in DNA replication: DNA polymerases ?, ?, and ? replicate NUCLEAR DNA. There is also a fourth DNA polymerase known as ?. What do you suppose is the specific role of DNA polymerase ?? DNA Pol ? is associated with primase activity. What is the role of primase in DNA replication? POLYMERASES INCLUDED POLYMERASES BOUND to DNA ?, ? ? ?, ? ? ?, ? ?, ? ?, ?, ? ?, ?, ? You are curious how eukaryotic DNA polymerases assemble at the replication origin. To study this you carry out experiments where you leave out a DNA polymerase and measure which polymerases bind the DNA. What can you conclude about the order of polymerase assembly at the origin? Explain your answer. POLYMERASES INCLUDED POLYMERASES BOUND to DNA Low salt High salt ?, ? ? none ?, ? ? none ?, ? ?, ? none ?, ?, ? ?, ?, ? ?, ?, ? The same experiment yields different results when you add NaCl to the reaction as shown on the far right column. What are some possible explanations for this observation? What does this finding suggest about the mechanism of polymerase assembly?

          Prokaryotic cells have 3 DNA polymerases: DNA Pol I, II, and III. How are these similar to each other and how are they different?

Eukaryotic cells have 4 DNA polymerases involved in DNA replication: DNA polymerases ?, ?, and ? replicate NUCLEAR DNA. There is also a fourth DNA polymerase known as ?. What do you suppose is the specific role of DNA polymerase ??

DNA Pol ? is associated with primase activity. What is the role of primase in DNA replication?

POLYMERASES INCLUDED
POLYMERASES BOUND to DNA
?, ?
?
?, ?
?
?, ?
?, ?
?, ?, ?
?, ?, ?

You are curious how eukaryotic DNA polymerases assemble at the replication origin. To study this you carry out experiments where you leave out a DNA polymerase and measure which polymerases bind the DNA. What can you conclude about the order of polymerase assembly at the origin? Explain your answer.

POLYMERASES INCLUDED
POLYMERASES BOUND to DNA
Low salt
High salt
?, ?
?
none
?, ?
?
none
?, ?
?, ?
none
?, ?, ?
?, ?, ?
?, ?, ?

The same experiment yields different results when you add NaCl to the reaction as shown on the far right column. What are some possible explanations for this observation? What does this finding suggest about the mechanism of polymerase assembly?

Prokaryotic cells have 3 DNA polymerases: DNA Pol I, II, and III. How are these similar to each other and how are they different?

Eukaryotic cells have 4 DNA polymerases involved in DNA replication: DNA polymerases ?, ?, and ? replicate NUCLEAR DNA. There is also a fourth DNA polymerase known as ?. What do you suppose is the specific role of DNA polymerase ??

DNA Pol ? is associated with primase activity. What is the role of primase in DNA replication?

POLYMERASES INCLUDED
POLYMERASES BOUND to DNA
?, ?
?
?, ?
?
?, ?
?, ?
?, ?, ?
?, ?, ?

You are curious how eukaryotic DNA polymerases assemble at the replication origin. To study this you carry out experiments where you leave out a DNA polymerase and measure which polymerases bind the DNA. What can you conclude about the order of polymerase assembly at the origin? Explain your answer.

POLYMERASES INCLUDED
POLYMERASES BOUND to DNA
Low salt
High salt
?, ?
?
none
?, ?
?
none
?, ?
?, ?
none
?, ?, ?
?, ?, ?
?, ?, ?

The same experiment yields different results when you add NaCl to the reaction as shown on the far right column. What are some possible explanations for this observation? What does this finding suggest about the mechanism of polymerase assembly?

Added by Barbara D.

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