Protocol 3. Using the formula above for estimating the size...

Protocol 3. Using the formula above for estimating the size of a microscopic organim and the compound light microscope image of a euglena below (Figure 4.) (400x FOVd = .5mm)Calculate the following sizes of the eyespot, euglena, flagella in mm ( millimeters) and um (nanometers). There is a metric conversion chart posted in the general lab information module for this course : 1.What is the approximate size of the euglena’s eyespot? ________ mm, ________ um 2. What is the approximate size of the euglena? ________ mm, ________ um 3. What is the approximate size of the euglenas flagella? ________ mm, ________ um Eye spot flagella Fig 4. Euglena viewed with a compound light microscope at 400x

Transcript

00:01 So what you're looking at, there is a field of view of 4x objective lens.

00:09 So this circular field is called the field of view.

00:13 Now the diameter, according to the question, equals 0 .5 millimeter.

00:22 So this is what we know.

00:25 So we are going to compare the size of each item in below, including the euglina's eye spot, the euglina itself, and the flagella of the euglina, and then use the field of view as a guideline to actually estimate the size of each of the item.

00:46 So let's start from the first one.

00:49 So i measure the figure itself using a ruler, and i find out that the diameter from measuring is about 4 .5 centimeter by using my ruler.

01:04 Now i also use my ruler to measure the first one, the eye spot will lead up.

01:15 So the size of a eye spot is about 0 .64 centimeter.

01:31 So this means we can actually calculate how many eyespots can fit across the diameter.

01:48 So the number of eyespot across is going to be the diameter 4 .5 centimeter divided by 0 .64 centimeter.

01:58 So we got around 7.

02:00 So we can fit 7 eyespots across the field will view the diameter.

02:05 But we also know the diameter from information actually equals 0 .5 millimeter.

02:11 So we get the ratio between the diameter and eye spot, and then we sort of estimate the size of the eye spot by doing the math.

02:23 So we know that seven eye spot can go across the diameter.

02:29 So size of each eye spot equals the total length, d, divide by seven.

02:38 So each eye spot is around one seventh of the diameter.

02:42 So 0 .5 millimeter divide by 7 equals around 0 .071 millimeter.

02:58 Now we also know that 1 millimeter equals 1 ,000 micrometer.

03:05 So you times 1 ,000 here to convert it into micrometer, which is 71 micrometer...

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