Provide the first 20 nucleotides of the genomic DNA sequence for the Caden.05G063200 gene from the American chestnut genome, using the Castanea dentata v1.1 reference genome sequence. Please provide all sequences in the 5' to 3' direction, in accordance with scientific convention. Provide the sequence only (i.e., do not indicate 5' or 3' in your answer).
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05G063200, in the American chestnut genome using the Castanea dentata v1.1 reference genome. Show more…
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For this exercise, you will need to access GenBank by going to the NCBI website and using the drop-down menu to search "Nucleotide." Note that the definition of the coding strand is the strand of DNA within the gene that is identical to the transcript (for genetic code, see Fig: 1.11). On the other hand, the template strand is the strand that is complementary to the coding strand. a. Use the following accession number to access the nucleotide sequence in GenBank: CU329670. b. Go to the FEATURES section of the record. c. Link to the CDS to gain access to the first 5662 nucleotides of the sequence. d. Name the protein product of the CDS. e. Write the first four amino acids (starting from the N-terminus). f. Write the nucleotide sequence of the coding strand that corresponds to these amino acids. g. Write the nucleotide sequence of the template strand that corresponds to these amino acids. h. Using the sequence shown in the record, give the nucleotide number range that corresponds to these amino acids.
Sulav P.
A1. The following is the DNA sequence of a hypothetical gene for the SHORTIE protein. It is called the SHORTIE gene. 1 atgccttacc ttaggctacg tccaaatagc cttcattgta 41 acaaggatc atag Answer A, B, C, and D. A. Design a 20 base reverse PCR primer you could use to amplify the entire SHORTIE gene sequence. Write the primer in your answer book, indicating the 5' and 3' ends of the sequence. B. Assume the sequence shown is the coding strand. If an mRNA was made from this sequence, what is the sequence of the template strand? Write the sequence in your answer book, indicating the 5' and 3' ends of the sequence. C. Assume the first three bases of the SHORTIE gene sequence shown are the start codon (ATG) and the last three bases are a stop codon (TAG). How many amino acid residues are in the encoded protein? Explain your answer. D. What is the name of an online bioinformatics tool you could use to quickly translate this sequence in 6 frames?
Supreeta N.
The following is an image of a Sanger sequencing gel. Based on this gel, what is the sequence of the DNA being sequenced? Please write it in 5'-to-3' direction (only use letters A, C, G, Twith no spaces)
Suman K.
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