Question

The sequence below represents a portion of the non-template strand of the human BRCA1 gene. The first nucleotide represents the start of the BRCA1 open reading frame. Every tenth nucleotide in the sequence is numbered and a large part of the intron sequence has been omitted to save space. 5'… ATG GAT TTA TCT GCT CTT CGC GTT GAA GAA GTA 1 10 20 30 CAA AAT GTC ATT AAT GCT ATG CAG AAA ATC TTA GAG 40 50 60 intron _____________________________________ TGT CCC ATC TGgtaagtcagc…….taccctgctagT CTG 70 80 GAG TTG ATC AAG GAA CCT GTC TCC ACA AAG TGT …3' 90 100 110 Six different human mutations in the BRCA1 gene are listed below. The sequences described in these mutations represent the non-template strand of DNA. Each of these mutations has been linked to breast and/or ovarian cancer.Classify each type of mutation and determine the effect on the amino acid sequence of BRCA1. Q2.1 Mutation 1 0.5 Points Remove nucleotide 69 (G) Type of mutation (select one): ? Silent ? Missense ? Nonsense ? Frameshift ? Mutated start codon ? Mutated stop codon ? In-frame insertion/deletion Effect on amino acid sequence (select one): ? A different start codon will be used ? A portion of the protein sequence before the mutation will be read correctly, but all codons after the mutation will be misread ? A single amino acid will be changed ? Multiple amino acids will be deleted without impacting the start or stop codon used ? The protein sequence will be read correctly up until the stop codon, but a different stop codon will be used ? Multiple amino acids will be added without impacting the start or stop codon used

The sequence below represents a portion of the non-template strand of the human BRCA1 gene. The first nucleotide represents the start of the BRCA1 open reading frame. Every tenth nucleotide in the sequence is numbered and a large part of the intron sequence has been omitted to save space.

5'… ATG GAT TTA TCT GCT CTT CGC GTT GAA GAA GTA
1 10 20 30

CAA AAT GTC ATT AAT GCT ATG CAG AAA ATC TTA GAG
40 50 60

intron
_____________________________________
TGT CCC ATC TGgtaagtcagc…….taccctgctagT CTG
70 80

GAG TTG ATC AAG GAA CCT GTC TCC ACA AAG TGT …3'
90 100 110

Six different human mutations in the BRCA1 gene are listed below. The sequences described in these mutations represent the non-template strand of DNA. Each of these mutations has been linked to breast and/or ovarian cancer.Classify each type of mutation and determine the effect on the amino acid sequence of BRCA1.

Q2.1 Mutation 1
0.5 Points

Remove nucleotide 69 (G)

Type of mutation (select one):
? Silent
? Missense
? Nonsense
? Frameshift
? Mutated start codon
? Mutated stop codon
? In-frame insertion/deletion

Effect on amino acid sequence (select one):
? A different start codon will be used
? A portion of the protein sequence before the mutation will be read correctly, but all codons after the mutation will be misread
? A single amino acid will be changed
? Multiple amino acids will be deleted without impacting the start or stop codon used
? The protein sequence will be read correctly up until the stop codon, but a different stop codon will be used
? Multiple amino acids will be added without impacting the start or stop codon used

The sequence below represents a portion of the non-template strand of the human BRCA1 gene. The first nucleotide represents the start of the BRCA1 open reading frame. Every tenth nucleotide in the sequence is numbered and a large part of the intron sequence has been omitted to save space.

5'… ATG GAT TTA TCT GCT CTT CGC GTT GAA GAA GTA
1 10 20 30

CAA AAT GTC ATT AAT GCT ATG CAG AAA ATC TTA GAG
40 50 60

intron

TGT CCC ATC TGgtaagtcagc…….taccctgctagT CTG
70 80

GAG TTG ATC AAG GAA CCT GTC TCC ACA AAG TGT …3'
90 100 110

Six different human mutations in the BRCA1 gene are listed below. The sequences described in these mutations represent the non-template strand of DNA. Each of these mutations has been linked to breast and/or ovarian cancer.Classify each type of mutation and determine the effect on the amino acid sequence of BRCA1.

Q2.1 Mutation 1
0.5 Points

Remove nucleotide 69 (G)

Type of mutation (select one):
? Silent
? Missense
? Nonsense
? Frameshift
? Mutated start codon
? Mutated stop codon
? In-frame insertion/deletion

Effect on amino acid sequence (select one):
? A different start codon will be used
? A portion of the protein sequence before the mutation will be read correctly, but all codons after the mutation will be misread
? A single amino acid will be changed
? Multiple amino acids will be deleted without impacting the start or stop codon used
? The protein sequence will be read correctly up until the stop codon, but a different stop codon will be used
? Multiple amino acids will be added without impacting the start or stop codon used

Added by Chelsea K.

Question

Please give Ace some feedback