Q2 (18 points) You are studying how various proteins are affected by the cell cycle. In each of the following experiments, you isolate certain populations of cells, and then analyze the relative DNA content of those cells by flow cytometry, obtaining the following 4 graphs.
Match each of the experiments below with the graph that best represents how your data would look. (Write the letter corresponding to the correct experiment in the box under the appropriate graph.)
A. You do a control experiment where you include all living cells from your culture
B. You isolate only those cells expressing M-cyclin.
C. You add a compound to induce DNA damage, and then isolate cells expressing high levels of p53.
Q3 (8 points) You have isolated diploid germ cells and grown them in a petri dish. Next, you add a drug that triggers meiosis to generate haploid gametes, and it works! Congratulations, that is an impressive achievement. However, you notice there might be something wrong with some of the gametes. You use DNA karyotyping to determine the number of chromosomes in each gamete. You notice that 50% of the gametes have an extra chromosome, and 50% of the gametes have one fewer chromosome than they should. Which of the following options do you suspect is the most likely cause of the defect you observe? (Circle the correct answer).
A. A chromosome is lost in the diploid germ cells
B. A chromosome is lost after meiosis I
C. A chromosome is lost after meiosis II
D. There is an error in chromosome segregation during meiosis I
E. There is an error in chromosome segregation during meiosis II