Question

Q4. A novel mutation in aquaporin 2, a water channel that is highly expressed in the collecting duct of the kidney, has recently been described. The figure illustrates the effect of expressing the mutant aquaporin (AQP2-Mutant) on water permeability (i.e., the rate of water movement) in MDCK cells in the presence (+ADH) or absence (-ADH) of antidiuretic hormone relative to cells expressing wildtype (normal) aquaporin (AQP2-WT). MDCK cells are a canine kidney cell line commonly used in biomedical research. Water Permeability (um/sec) 60 AQP2-WT AQP2-Mutant 40 20 0 -ADH +ADH -ADH +ADH A. What effect does the mutated aquaporin have on water permeability? B. What effect would this mutation have on urine volume (would it increase, decrease, or stay the same)? Explain your answer. C. What pathophysiological condition is caused by this mutation?

          Q4. A novel mutation in aquaporin 2, a water channel that is highly expressed
in the collecting duct of the kidney, has recently been described. The figure
illustrates the effect of expressing the mutant aquaporin (AQP2-Mutant) on
water permeability (i.e., the rate of water movement) in MDCK cells in the
presence (+ADH) or absence (-ADH) of antidiuretic hormone relative to cells
expressing wildtype (normal) aquaporin (AQP2-WT). MDCK cells are a canine
kidney cell line commonly used in biomedical research.
Water Permeability
(um/sec)
60
AQP2-WT
AQP2-Mutant
40
20
0
-ADH +ADH -ADH +ADH
A. What effect does the mutated aquaporin have on water permeability?
B. What effect would this mutation have on urine volume (would it increase, decrease, or stay the same)? Explain
your answer.
C. What pathophysiological condition is caused by this mutation?

Q4. A novel mutation in aquaporin 2, a water channel that is highly expressed
in the collecting duct of the kidney, has recently been described. The figure
illustrates the effect of expressing the mutant aquaporin (AQP2-Mutant) on
water permeability (i.e., the rate of water movement) in MDCK cells in the
presence (+ADH) or absence (-ADH) of antidiuretic hormone relative to cells
expressing wildtype (normal) aquaporin (AQP2-WT). MDCK cells are a canine
kidney cell line commonly used in biomedical research.
Water Permeability
(um/sec)
60
AQP2-WT
AQP2-Mutant
40
20
0
-ADH +ADH -ADH +ADH
A. What effect does the mutated aquaporin have on water permeability?
B. What effect would this mutation have on urine volume (would it increase, decrease, or stay the same)? Explain
your answer.
C. What pathophysiological condition is caused by this mutation?

Added by John N.

Question

Please give Ace some feedback