Question

Q5 2 Points The diagram below represents the structure of the Lucky gene, which is expressed in every cell in the fruit fly. Gray boxes show exons; box sizes are not to scale. Lucky is alternatively spliced, with exon 2 skipped in all cells in the head. Assume that poly-A polymerase adds 200 nucleotides to each transcript. Promoter Translation Start Site (+101-+103) +200+201 +1200 +1201+1400+1401+3400+3401 +3850+3851 +3901 Stop Codon PolyA (+3701-+3703) site Transcription Termination Site Q5.1 1 Point On the gel below, draw how the northern blot will look when mRNA is isolated from the whole fly, or only from the head, using a probe complementary to a portion of the mRNA in exon 1 (shown in the diagram above). Label the band sizes next to the gel. mRNA from whole flies mRNA from head only Please select file(s) Select file(s) Save Answer Q5.2 1 Point Your labmate designs a different RNA probe for the Lucky gene, fluorescently labels it, and uses it in in situ hybridization. The probe shows fluorescence in the entire fly except for in the head. Where in the gene is the probe that your labmate designed?

          Q5
2 Points
The diagram below represents the structure of the Lucky gene, which is expressed in
every cell in the fruit fly. Gray boxes show exons; box sizes are not to scale. Lucky is alternatively
spliced, with exon 2 skipped in all cells in the head. Assume that poly-A polymerase adds 200
nucleotides to each transcript.
Promoter
Translation
Start Site
(+101-+103)
+200+201 +1200 +1201+1400+1401+3400+3401
+3850+3851 +3901
Stop Codon PolyA
(+3701-+3703) site
Transcription
Termination Site
Q5.1
1 Point
On the gel below, draw how the northern blot will look when mRNA is isolated from the whole fly,
or only from the head, using a probe complementary to a portion of the mRNA in exon 1 (shown in
the diagram above). Label the band sizes next to the gel.
mRNA from
whole flies
mRNA from
head only
Please select file(s) Select file(s)
Save Answer
Q5.2
1 Point
Your labmate designs a different RNA probe for the Lucky gene, fluorescently labels it, and uses it
in in situ hybridization. The probe shows fluorescence in the entire fly except for in the head. Where
in the gene is the probe that your labmate designed?

Q5
2 Points
The diagram below represents the structure of the Lucky gene, which is expressed in
every cell in the fruit fly. Gray boxes show exons; box sizes are not to scale. Lucky is alternatively
spliced, with exon 2 skipped in all cells in the head. Assume that poly-A polymerase adds 200
nucleotides to each transcript.
Promoter
Translation
Start Site
(+101-+103)
+200+201 +1200 +1201+1400+1401+3400+3401
+3850+3851 +3901
Stop Codon PolyA
(+3701-+3703) site
Transcription
Termination Site
Q5.1
1 Point
On the gel below, draw how the northern blot will look when mRNA is isolated from the whole fly,
or only from the head, using a probe complementary to a portion of the mRNA in exon 1 (shown in
the diagram above). Label the band sizes next to the gel.
mRNA from
whole flies
mRNA from
head only
Please select file(s) Select file(s)
Save Answer
Q5.2
1 Point
Your labmate designs a different RNA probe for the Lucky gene, fluorescently labels it, and uses it
in in situ hybridization. The probe shows fluorescence in the entire fly except for in the head. Where
in the gene is the probe that your labmate designed?

Added by Manuela D.

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