A neurophysiology expert is teaching his students the physiology of the neuromuscular junction. While describing the sequence of events that takes place at the neuromuscular junction, he mentions that as the action potential travels down the motor neuron, it produces depolarization of the presynaptic membrane. This results in the opening of voltage-gated calcium channels, which leads to the release of calcium ions into the synaptic cleft. The binding of calcium ions to nicotinic acetylcholine receptors on the motor end plate causes an increased conductance of sodium and potassium ions. Additionally, calcium ions bind to vesicles containing acetylcholine, leading to exocytosis of acetylcholine into the synaptic cleft. This process ultimately results in the generation of an end plate potential.