Many proteins are involved in DNA replication. Which of the following correctly identifies the function of a protein involved in DNA replication? Choose both that apply Ligase breaks hydrogen bonds between the two strands. Topoisomerase relieves tension at the replication fork. SSBPs hold the two strands apart once they have been separated. Primase holds DNA polymerase to the strand during replication. DNA polymerase I adds a primer to the strand to initiate replication. DNA polymerase III replaces RNA primers with deoxyribonucleotides.
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Topoisomerase relieves tension ahead of the replication fork. Show more…
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3. a) List the function of each of the following 7 proteins during DNA replication, and b) Number each protein "1" through "7" to describe which acts first during replication. _____ Single-strand binding proteins (SSBPs) _____ DNA polymerase III (DNA pol III) _____ Helicase _____ RNA Primase _____ Topoisomerase _____ DNA ligase _____ DNA polymerase I (DNA pol I) 4. Draw a replication fork and label it with the following: a. 5' and 3' ends of your DNA: label each strand - old and new b. Leading and lagging strands c. Helicase, SSBPs, DNA pol I, DNA pol III, DNA ligase, RNA Primase, Topoisomerase d. Okazaki fragments
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Label each spontaneous mutation or mutagen as causing a base-substitution, addition/deletion of trinucleotide repeats, frameshift, or breaks in DNA. DNA polymerase adds a non-complementary base during DNA replication base-substitution Strand slippage addition/deletion of trinucleotide repeats Depurination base-substitution Deamination base-substitution Nucleotide base analogs base-substitution Deaminating agents base-substitution Alkylating agents breaks in DNA Oxidizing agents breaks in DNA Hydroxylating agents base-substitution Intercalating agents frameshift UV radiation frameshift
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